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CRISPR / Cas9系统是用于基因编辑的RNA引导核酸内切酶技术。该系统需要包含与PAM（Protospacer Adjacent Motif）位点相邻的20bp靶序列的指导RNA（gRNA），以将Cas9核酸内切酶引导至切割位点。Geneious中的Find CRISPR网站工具将搜索所选基因中的gRNA（“CRISPR”）位点，并在您感兴趣的基因组中搜索脱靶结合位点。选定序列中的每个CRISPR站点根据它可能会绑定到多少个脱离目标站点以及脱离目标站点与原始序列的相似程度进行评分。
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